Project Overview
Project Description
Fused deposition modeling (FDM) is a scaffold fabrication technique that utilizes 3D printing as a means for extruding polymer microfibers into a designed architecture. The design of the FDM BioPrinter includes Atmega processing of X,Y,Z coordinates and a proportional-integral-derivative (PID) temperature controller. Automation of processes of extrusion, chemical sterilization, and cell deposition limits user interaction to minimize risk of contamination. These processes create scaffolds with three-dimensional, customizable architectures that promote fibroblast adhesion and proliferation. Preliminary testing validated the sterilization procedure of the FDM BioPrinter, as cells grew to confluence and no contamination was observed after two weeks. Final testing of the device includes quantitative assays for cell proliferation and microscopic evaluation of scaffold architectures.
To address the donor shortage for tissue and organ transplants, researchers have developed cell-based substitutes that restore function. For tissue constructs, one approach incorporates cell seeding onto a polymer scaffold and introduction of essential biochemicals to support cell adhesion and proliferation. Ideal scaffold characteristics include bioresorbability, interconnectivity, and a high surface area/volume ratio.
A novel scaffold fabrication technique known as fused deposition modeling (FDM) is characterized by continuous layer-by-layer extrusion of a thermoplastic polymer filament. Advantages of FDM include its exclusion of an organic solvent, rapid solidification of the extruded polymer, and the structural integrity of the resulting three-dimensional matrix.The objective of the FDM BioPrinter is to create customizable, reproducible scaffolds that support a uniform cell distribution. A computer-guided, movable build platform minimizes user contact throughout the following automated process:
To address the donor shortage for tissue and organ transplants, researchers have developed cell-based substitutes that restore function. For tissue constructs, one approach incorporates cell seeding onto a polymer scaffold and introduction of essential biochemicals to support cell adhesion and proliferation. Ideal scaffold characteristics include bioresorbability, interconnectivity, and a high surface area/volume ratio.
A novel scaffold fabrication technique known as fused deposition modeling (FDM) is characterized by continuous layer-by-layer extrusion of a thermoplastic polymer filament. Advantages of FDM include its exclusion of an organic solvent, rapid solidification of the extruded polymer, and the structural integrity of the resulting three-dimensional matrix.The objective of the FDM BioPrinter is to create customizable, reproducible scaffolds that support a uniform cell distribution. A computer-guided, movable build platform minimizes user contact throughout the following automated process:
Group Members
Hayin Candiotti
Brian Karl
Kendra Knowles
Dana Mathews
Kyle Mohen
Brian Karl
Kendra Knowles
Dana Mathews
Kyle Mohen
Advisers
Dr. Constance Hall
Associate Professor of Biomedical Engineering, Department Chair
University of Memphis (PhD, 1995)
Dr. Manish Paliwal
Associate Professor of Mechanical and Biomedical Engineering
Southern Illinois University at Carbondale (PhD, 2003)
Associate Professor of Biomedical Engineering, Department Chair
University of Memphis (PhD, 1995)
Dr. Manish Paliwal
Associate Professor of Mechanical and Biomedical Engineering
Southern Illinois University at Carbondale (PhD, 2003)