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Preliminary Testing
METHODS
To simulate and validate the sterilization process through evaluation of cell proliferation, 12 segments of PCL filament were placed in a 24-well plate and washed with ethanol and PBS. Six of the twelve segments were seeded with BALB/3T3 Clone A31 Mouse Embryo Fibroblasts (ATCC) by hand, and then cultured in complete growth medium in an incubator (37°C, 5.0% CO2).
RESULTS
Observation of cell growth over a 14-day period confirmed that contamination did not occur. Cells were then trypsinized from the filament, transferred to a T-25 flask, and proliferated to confluence for two weeks.
METHODS
To simulate and validate the sterilization process through evaluation of cell proliferation, 12 segments of PCL filament were placed in a 24-well plate and washed with ethanol and PBS. Six of the twelve segments were seeded with BALB/3T3 Clone A31 Mouse Embryo Fibroblasts (ATCC) by hand, and then cultured in complete growth medium in an incubator (37°C, 5.0% CO2).
RESULTS
Observation of cell growth over a 14-day period confirmed that contamination did not occur. Cells were then trypsinized from the filament, transferred to a T-25 flask, and proliferated to confluence for two weeks.