Cell Seeding into PCL Scaffold
Cell deposition is a critical aspect during the fabrication of a tissue constructs. Numerous techniques have been utilized for cell deposition in tissue engineering research including filtration, ink-jet, pneumatic, and micro-syringe. Different deposition methods have been investigated to reduce the required cell concentration, experimental length, and increase distribution though a 3D scaffold. The latter of which has been found to influences the mechanical strength and cell proliferation.
The FDM Bioprinter was designed to be highly versatile as in it can handle multiple cell types and scaffold geometries. For this device a syringe pump was chosen as the method to deposit the cells. This allows for the flow rate and location of dispensation to be controlled by a computer. Additionally it provides functionality by integration with the rotating chamber. This allows for the syringe pump to be used for sterilization of the scaffold as well as cell deposition.
The FDM Bioprinter was designed to be highly versatile as in it can handle multiple cell types and scaffold geometries. For this device a syringe pump was chosen as the method to deposit the cells. This allows for the flow rate and location of dispensation to be controlled by a computer. Additionally it provides functionality by integration with the rotating chamber. This allows for the syringe pump to be used for sterilization of the scaffold as well as cell deposition.
Brian Karl is responsible for this aspect of the project